Journal Article

Within-tree transcriptome profiling in wood-forming tissues of a fast-growing <i>Eucalyptus tree</i>

Martin Ranik, Nicky M. Creux and Alexander A. Myburg

in Tree Physiology

Volume 26, issue 3, pages 365-375
Published in print March 2006 | ISSN: 0829-318X
Published online March 2006 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/26.3.365
Within-tree transcriptome profiling in wood-forming tissues of a fast-growing Eucalyptus tree

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Despite the availability of high-throughput transcript profiling technology, little is known about tissue-specific gene expression patterns in the wood-forming tissues of Eucalyptus plantation tree species. We used cDNA-amplified fragment length polymorphism (AFLP) analysis in combination with infrared fragment detection and semi-automated band quantification to profile gene expression in a 6-year-old, fast-growing Eucalyptus tree. The expression profiles of 6385 transcript-derived fragments (TDFs) were analyzed across four major woody tissues (mature xylem, immature xylem, phloem and cork) collected from two stem positions, to provide a global view of transcript abundance and variability in the Eucalyptus stem. About 21% of the TDFs were differentially expressed and could be grouped into clusters representing co-expressed genes. A total of 71 TDFs representing different gene clusters were isolated and characterized. These included genes implicated in cell fate, signal transduction and cell wall biosynthesis, processes closely associated with xylogenesis. Analysis of the expression levels of selected TDFs by quantitative RT-PCR corroborated the TDF quantification and confirmed that cDNA-AFLP analysis is a highly efficient and accurate tool for transcript profiling and gene discovery in wood-forming tissues of tree species.

Keywords: cambium; cDNA-AFLP; cork; gene expression; phloem; plantation forests; quantitative RT-PCR; transcriptomics; xylem; xylogenesis

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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