Journal Article

Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO<sub>2</sub> concentration

Jana Cabálková, Jan Přibyl, Petr Skládal, Pavel Kulich and Josef Chmelík

in Tree Physiology

Volume 28, issue 10, pages 1593-1599
Published in print August 2008 | ISSN: 0829-318X
Published online August 2008 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/28.10.1593
Size, shape and surface morphology of starch granules from Norway spruce needles revealed by transmission electron microscopy and atomic force microscopy: effects of elevated CO2 concentration

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Summary

We compared the effects of ambient (350 ppm) and elevated CO2 concentration (700 ppm) on the size and shape of starch granules in Norway spruce (Picea abies (L.) Karst.) needles during one growing season. Starch granules were isolated from needles by alkaline digestion and analyzed by transmission electron microscopy (TEM) and atomic force microscopy (AFM). Measurements made with a particle size analyzer indicated that starch granules ranged between 0.5 and 10 μm. Granule size and shape varied according to needle developmental stage and CO2 concentration. Generally, elevated CO2 concentration increased the size of the starch granules. Fine surface structures (< 10 nm in size) studied by AFM were characterized by the presence of protrusions, furrows and pores.

Keywords: AFM; Picea abies; starch granules; TEM

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Subjects: Plant Sciences and Forestry

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