Journal Article

Microspore development of three coniferous species: affinity of nuclei for flavonoids

Walter Feucht, Dieter Treutter, Heike Dithmar and Jürgen Polster

in Tree Physiology

Volume 28, issue 12, pages 1783-1791
Published in print October 2008 | ISSN: 0829-318X
Published online October 2008 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/28.12.1783
Microspore development of three coniferous species: affinity of nuclei for flavonoids

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Summary

The nuclear localization of blue-staining flavanols was investigated histochemically throughout microsporogenesis in yellow cypress (Callitropsis nootkatensis (D. Don) Oerst., formerly Cupressus nootkatensis), juniper (Juniperus communis L.) and yew (Taxus baccata L.). During meiotic development, both the cytoplasm and nuclei of microspores of all species contained varying amounts of flavanols; however, the flavanols were largely confined to the nuclei in microspores just released from tetrads. Quantification by HPLC analysis indicated that, in all species, catechin and epicatechin were the dominant nuclear flavanols. At the early free microspore stage, the nuclear flavanols were barely detectable in all species, but they increased fivefold on incubation in the presence of 0.1 mM benzylaminopurine (BA) or zeatin. Histochemical studies revealed that, in addition to non-fluorescing flavanols, microspores contained yellow-fluorescing flavonoids, which yielded a distinct HPLC flavonoid profile for each species. In yellow cypress, the hydrolyzed flavonoids were identified as quercetin, apigenin, kaempferol and luteolin, whereas only quercetin and myricetin were found in microspores of juniper and in anthers of yew. Application of a UV-VIS titration technique revealed that the aglycone quercetin seems to interact more strongly with histone H3 than either glycoside rutin or kaempferol.

Keywords: cytokinins; histology; histones; HPLC; UV-VIS spectrometric titrations

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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