Journal Article

SAGE transcript profiling of the juvenile cambial region of <i>Eucalyptus grandis</i>

Mayra Costa da Cruz Gallo de Carvalho, Danielle Gregorio Gomes Caldas, Raphael Tozelli Carneiro, David Henry Moon, Guillermo Rafael Salvatierra, Lívia Maria Franceschini, Alexander de Andrade, Paola Alejandra Fiorani Celedon, Shinitiro Oda and Carlos Alberto Labate

in Tree Physiology

Volume 28, issue 6, pages 905-919
Published in print June 2008 | ISSN: 0829-318X
Published online June 2008 | e-ISSN: 1758-4469 | DOI:
SAGE transcript profiling of the juvenile cambial region of Eucalyptus grandis

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Despite the importance of Eucalyptus spp. in the pulp and paper industry, functional genomic approaches have only recently been applied to understand wood formation in this genus. We attempted to establish a global view of gene expression in the juvenile cambial region of Eucalyptus grandis Hill ex Maiden. The expression profile was obtained from serial analysis of gene expression (SAGE) library data produced from 3- and 6-year-old trees. Fourteen-base expressed sequence tags (ESTs) were searched against public Eucalyptus ESTs and annotated with GenBank. Altogether 43,304 tags were generated producing 3066 unigenes with three or more copies each, 445 with a putative identity, 215 with unknown function and 2406 without an EST match. The expression profile of the juvenile cambial region revealed the presence of highly frequent transcripts related to general metabolism and energy metabolism, cellular processes, transport, structural components and information pathways. We made a quantitative analysis of a large number of genes involved in the biosynthesis of cellulose, pectin, hemicellulose and lignin. Our findings provide insight into the expression of functionally related genes involved in juvenile wood formation in young fast-growing E. grandis trees.

Keywords: cell wall; cellulose; gene expression; lignin; nucleotide-sugars interconvertions; transcriptome; wood formation

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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