Journal Article

Molecular cloning and expression analysis of a gene encoding a putative β-ketoacyl-acyl carrier protein (ACP) synthase III (KAS III) from <i>Jatropha curcas</i>

Jun Li, Mei-Ru Li, Ping-Zhi Wu, Chang-En Tian, Hua-Wu Jiang and Guo-Jiang Wu

in Tree Physiology

Volume 28, issue 6, pages 921-927
Published in print June 2008 | ISSN: 0829-318X
Published online June 2008 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/28.6.921
Molecular cloning and expression analysis of a gene encoding a putative β-ketoacyl-acyl carrier protein (ACP) synthase III (KAS III) from Jatropha curcas

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A cDNA clone encoding a putative β-ketoacyl-acyl carrier protein (ACP) synthase III (KAS III) was isolated from Jatropha curcas L., a woody oil plant. The cDNA clone (named JcKAS III) contained a 1203-bp open reading frame coding for 400 amino acids with a predicted molecular mass of about 42 kDa. The deduced amino acid sequence of the cDNA clone shares about 80% identity to KAS III from other plants, and contains a conserved Cys176 in the active site and the amino acid motif G355NTSAAS361 which is responsible for binding regulatory acyl-ACPs. Southern blotting analysis indicated that JcKAS III is a single copy gene in the J. curcas genome. Quantitative real-time PCR analysis showed that JcKAS III was expressed in all tissues examined with highest expression in roots, and that expression of JcKAS III increased as seeds developed.

Keywords: carbon chain elongation; fatty acid synthesis; seed oil

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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