Journal Article

Identification and quantitative analysis of stage-specific carbohydrates in loblolly pine (<i>Pinus taeda</i>) zygotic embryo and female gametophyte tissues

Gerald S. Pullman and Mike Buchanan

in Tree Physiology

Volume 28, issue 7, pages 985-996
Published in print July 2008 | ISSN: 0829-318X
Published online July 2008 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/28.7.985
Identification and quantitative analysis of stage-specific carbohydrates in loblolly pine (Pinus taeda) zygotic embryo and female gametophyte tissues

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Stage-specific analyses of starch and 18 sugars, including pentoses, hexoses, disaccharides, trisaccharides, oligosaccharides and sugar alcohols, were made throughout seed development for zygotic embryo and female gametophyte (FG) tissues of loblolly pine (Pinus taeda L.). Tissue was most often analyzed in triplicate from two open-pollinated families grown in different locations and sampled in different years. Carbohydrates were analyzed by enzymatic assay, high performance liquid chromatography or gas chromatography/mass spectrometry. For all carbohydrates quantified, peak concentrations were higher in embryo tissue than in FG tissue. Significant changes in starch and sugar concentrations occurred over time, with both seed collections showing similar trends in temporal changes. Although concentrations were not always similar, embryo and FG tissues generally showed similar patterns of change in starch and sugar concentrations over time. Total starch concentration was highest during early seed development and decreased as development progressed. The major sugars contributing to osmotic potential during early seed development were D-pinitol, sucrose, fructose and glucose. During mid-seed development, D-pinitol, sucrose, fructose, glucose, melibiose and raffinose provided major contributions to the osmotic environment. During late seed development, sucrose, raffinose, melibiose, stachyose and fructose were the major contributors to osmotic potential. These data suggest stage-specific media composition for each step in the somatic embryogenesis protocol.

Keywords: chemical analysis; conifer; embryogenesis; gymnosperm; megagametophyte; somatic embryogenesis; starch; sugar alcohol

Journal Article.  0 words. 

Subjects: Plant Sciences and Forestry

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