Journal Article

Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine

Hanna Shin, Hyoshin Lee, Kwan-Soo Woo, Eun-Woon Noh, Yeong-Bon Koo and Kyung-Joon Lee

in Tree Physiology

Volume 29, issue 3, pages 411-421
Published in print March 2009 | ISSN: 0829-318X
Published online March 2009 | e-ISSN: 1758-4469 | DOI:
Identification of genes upregulated by pinewood nematode inoculation in Japanese red pine

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Pine wilt disease caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus (Steiner et Buhrer) Nickle, has destroyed huge areas of pine forest in East Asia, including Japan, China and Korea. No protection against PWN has been developed, and the responses of pine trees at the molecular level are unrecorded. We isolated and analyzed upregulated or newly induced genes from PWN-inoculated Japanese red pine (Pinus densiflora Sieb. et Zucc.) by using an annealing control primer system and suppression subtractive hybridization. Significant changes occurred in the transcript abundance of genes with functions related to defense, secondary metabolism and transcription, as the disease progressed. Other gene transcripts encoding pathogenesis-related proteins, pinosylvin synthases and metallothioneins were also more abundant in PWN-inoculated trees than in non-inoculated trees. Our report provides fundamental information on the molecular mechanisms controlling the biochemical and physiological responses of Japanese red pine trees to PWN invasion.

Keywords: annealing control primer system; differentially expressed genes; pine wilt disease; Pinus densiflora; suppression subtractive hybridization

Journal Article.  6665 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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