Journal Article

Transcriptional changes in <i>CiFT</i>-introduced transgenic trifoliate orange (<i>Poncirus trifoliata</i> L. Raf.)

Fumie Nishikawa, Tomoko Endo, Takehiko Shimada, Hiroshi Fujii, Tokurou Shimizu, Yasushi Kobayashi, Takashi Araki and Mitsuo Omura

in Tree Physiology

Volume 30, issue 3, pages 431-439
Published in print March 2010 | ISSN: 0829-318X
Published online January 2010 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/tpp122
Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

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Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction analysis indicated that genes relating to transcription, cell wall modification and defense responses were expressed at higher levels in the transgenic shoots than in the control wild-type shoots. Of the genes showing ectopic mRNA accumulation, two SEPALLATA (SEP) and one FRUITFULL (FUL) homologues (CuSEP1, CuSEP3 or CuFUL) were introduced to Arabidopsis thaliana. Constitutive expression of each gene caused early flowering in transgenic Arabidopsis, suggesting that these genes could function as regulators of flowering time.

Keywords: citrus; flowering; Flowering Locus T (FT); FRUITFULL (FUL); SEPALLATA (SEP)

Journal Article.  4698 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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