Journal Article

Differential impact of chronic ozone exposure on expanding and fully expanded poplar leaves

Sacha Bohler, Kjell Sergeant, Isabelle Lefèvre, Yves Jolivet, Lucien Hoffmann, Jenny Renaut, Pierre Dizengremel and Jean-Francois Hausman

Edited by Marc Abrams

in Tree Physiology

Volume 30, issue 11, pages 1415-1432
Published in print November 2010 | ISSN: 0829-318X
Published online November 2010 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/tpq082
Differential impact of chronic ozone exposure on expanding and fully expanded poplar leaves

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Populus tremula L. × Populus alba L. (Populus×canescens (Aiton) Smith) — clone INRA 717-1-B4 saplings (50 cm apex to base and carrying 19 leaves on average) — were followed for 28 days. Half of the trees were grown in charcoal-filtered air while the other half were exposed to 120 ppb ozone for 11 h a day during the light period. The expanding leaf number 4 was tagged at the beginning of the experiment and finished expansion between 7 and 14 days. These leaves were harvested weekly for biochemical and proteome analyses using quantitative bidimensional electrophoresis (DiGE). Independent of the ozone treatment, all the analyses allowed a distinction between expanding and adult leaves. The results indicate that during the expansion phase (Days 0–7) the enzymatic machinery of the leaves is set up, and remains dynamically stable in the adult leaves (Days 14–28). Although ozone had no apparent effect on expanding leaves, the metabolic stability in fully expanded leaves observed in ozone-free plants was disturbed after 2 weeks of exposure and a stress-induced response became apparent.

Keywords: air pollution; biochemistry; difference in gel electrophoresis (DiGE); photosynthesis; primary carbon metabolism; proteomics

Journal Article.  8974 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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