Journal Article

Degradation of chloroplast DNA during natural senescence of maple leaves

Hrvoje Fulgosi, Marin Ježić, Hrvoje Lepeduš, Petra Peharec Štefanić, Mirna Ćurković-Perica and Vera Cesar

Edited by Jörg-Peter Schnitzler

in Tree Physiology

Volume 32, issue 3, pages 346-354
Published in print March 2012 | ISSN: 0829-318X
Published online March 2012 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/tps014
Degradation of chloroplast DNA during natural senescence of maple leaves

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The fate of chloroplast DNA (cpDNA) during plastid development and conversion between various plastid types is still not very well understood. This is especially true for the cpDNA found in plastids of naturally senescing leaves. Here, we describe changes in plastid nucleoid structure accompanied with cpDNA degradation occurring during natural senescence of the free-growing deciduous woody species Acer pseudoplatanus L. Natural senescence was investigated using three types of senescing leaves: green (G), yellow-green (YG) and yellow (Y). The extent of senescence was evaluated at the level of photosynthetic pigment degradation, accumulation of starch and plastid ultrastructure. Determination of cpDNA amount was carried out by in planta visualization with 4,6-diamidino-2-phenylindole, by Southern hybridization, and by dot-blot using an rbcL gene probe. During natural senescence, plastid nucleoids undergo structural rearrangements accompanied by an almost complete loss of cpDNA. Furthermore, senescence-associated protein components exhibiting strong binding to an ∼10kbp rbcL-containg cpDNA fragment were identified. This interaction might be important for rbcL expression and Rubisco degradation during the course of natural senescence in trees.

Keywords: deciduous plants; nucleases; nucleoids; quantification and arrangement of plastid DNA; rubisco regulation

Journal Article.  5554 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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