Journal Article

Observation of the scale of patchy stomatal behavior in leaves of <i>Quercus crispula</i> using an Imaging-PAM chlorophyll fluorometer

Mai Kamakura, Yoshiko Kosugi, Satoru Takanashi, Hiroyuki Tobita, Akira Uemura and Hajime Utsugi

Edited by Ülo Niinemets

in Tree Physiology

Volume 32, issue 7, pages 839-846
Published in print July 2012 | ISSN: 0829-318X
Published online June 2012 | e-ISSN: 1758-4469 | DOI: http://dx.doi.org/10.1093/treephys/tps053
Observation of the scale of patchy stomatal behavior in leaves of Quercus crispula using an Imaging-PAM chlorophyll fluorometer

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Patchy stomatal closure occurs in plants with heterobaric leaves, in which vertical extensions of bundle sheath cells delimit the mesophyll and restrict the diffusion of CO2. The scale of patchy stomatal behavior was investigated in this study. The distribution of PSII quantum yield (ΦII) obtained from chlorophyll fluorescence images was used to evaluate the scale of stomatal patchiness and its relationship with leaf photosynthesis in the sun leaves of 2-year-old saplings of Quercus crispula Blume. Fluorescent patches were observed only during the day with low stomatal conductance. Comparison of numerical simulation of leaf gas exchange and chlorophyll fluorescence images showed that heterogeneous distribution of electron transport rate through PSII (J) was observed following stomatal closure with a bimodal manner under both natural and saturated photosynthetic photon flux densities. Thus, fluorescence patterns can be interpreted in terms of patchy stomatal closure. The mapping of J from chlorophyll fluorescence images showed that the scale of stomatal patchiness was approximately 2.5-fold larger than that of anatomical patches (lamina areas bounded by bundle sheath extensions within lamina). Our results suggest the spatial scale of stomatal patches in Q. crispula leaves.

Keywords: chlorophyll fluorescence imaging; heterobaric leaf; midday depression of photosynthesis; patchy stomatal closure

Journal Article.  4437 words.  Illustrated.

Subjects: Plant Sciences and Forestry

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