Journal Article

Novel integrons and gene cassettes from a Cascadian submarine gas-hydrate-bearing core

Hosam Elsaied, Hatch W. Stokes, Hideyoshi Yoshioka, Yasuo Mitani and Akihiko Maruyama

in FEMS Microbiology Ecology

Volume 87, issue 2, pages 343-356
Published in print February 2014 |
Published online February 2014 | e-ISSN: 1574-6941 | DOI: http://dx.doi.org/10.1111/1574-6941.12227

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To determine whether integrons are present in a submarine gas hydrate community, metagenomic DNA was extracted from a gas-hydrate-bearing core, 150 m below the seafloor, from the Cascadian Margin. Integrons and gene cassettes were recovered by PCR from metagenomic DNA and sequenced. Thirty-seven integron integrase phylotypes were identified. The phylotypes were diverse and included members with homology to integrases from Methylomonas methanica, Desulfuromonas acetoxidans, Thermodesulfatator indicus, and marine uncultured bacteria. The gene cassette composition, 153 gene cassettes, was dominated by two types of encoded putative proteins. The first of these was predicted oxidoreductases, such as iron/sulfur cluster-binding proteins. A second type was alkyl transferases. Some cassette proteins showed homologies with those from methane-related archaea. These observations suggest that integrons may assist in the adaptation of microbial communities in this environment.

Keywords: integrons; gene cassettes; deep submarine gas hydrate; structure; diversity; lateral cassette gene transfer

Journal Article.  6362 words.  Illustrated.

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