Journal Article

Efficient use of DNA molecular markers to construct industrial yeast strains

Philippe Marullo, Gael Yvert, Marina Bely, Michel Aigle and Denis Dubourdieu

in FEMS Yeast Research

Volume 7, issue 8, pages 1295-1306
Published in print December 2007 |
Published online September 2007 | e-ISSN: 1567-1364 | DOI: http://dx.doi.org/10.1111/j.1567-1364.2007.00281.x

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Abstract

Saccharomyces cerevisiae yeast strains exhibit a huge genotypic and phenotypic diversity. Breeding strategies taking advantage of these characteristics would contribute greatly to improving industrial yeasts. Here we mapped and introgressed chromosomal regions controlling industrial yeast properties, such as hydrogen sulphide production, phenolic off-flavor and a kinetic trait (lag phase duration). Two parent strains derived from industrial isolates used in winemaking and which exhibited significant quantitative differences in these traits were crossed and their progeny (50–170 clones) was analyzed for the segregation of these traits. Forty-eight segregants were genotyped at 2212 marker positions using DNA microarrays and one significant locus was mapped for each trait. To exploit these loci, an introgression approach was supervised by molecular markers monitoring using PCR/RFLP. Five successive backcrosses between an elite strain and appropriate segregants were sufficient to improve three trait values. Microarray-based genotyping confirmed that over 95% of the elite strain genome was recovered by this methodology. Moreover, karyotype patterns, mtDNA and tetrad analysis showed some genomic rearrangements during the introgression procedure.

Keywords: genetic mapping; DNA microarray; QTL; introgression; breeding; wine yeast

Journal Article.  6428 words.  Illustrated.

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