Journal Article

Monitoring impact of in situ biostimulation treatment on groundwater bacterial community by DGGE

Tomotada Iwamoto, Katsuji Tani, Kanji Nakamura, Yoshihiko Suzuki, Masayoshi Kitagawa, Masahiro Eguchi and Masao Nasu

in FEMS Microbiology Ecology

Volume 32, issue 2, pages 129-141
Published in print April 2000 |
Published online January 2006 | e-ISSN: 1574-6941 | DOI:

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Changes in bacterial diversity during the field experiment on biostimulation were monitored by denaturing gradient gel electrophoresis (DGGE) analysis of PCR-amplified 16S rDNA fragments. The results revealed that the bacterial community was disturbed after the start of treatment, continued to change for 45 days or 60 days and then formed a relatively stable community different from the original community structure. DGGE analysis of soluble methane monooxygenase (sMMO) hydroxylase gene fragments, mmoX, was performed to monitor the shifts in the numerically dominant sMMO-containing methanotrophs during the field experiment. Sequence analysis on the mmoX gene fragments from the DGGE bands implied that the biostimulation treatment caused a shift of potential dominant sMMO-containing methanotrophs from type I methanotrophs to type II methanotrophs.

Keywords: Bacterial diversity; Denaturing gradient gel electrophoresis; In situ biostimulation; mmoX gene; Trichloroethylene degrading methanotrophs

Journal Article.  6405 words.  Illustrated.

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