Journal Article

<i>Mycobacterium tuberculosis</i> reactive T cell clones from naturally converted PPD-positive healthy subjects: recognition of the <i>M. tuberculosis</i> 16-kDa antigen

Fredrik Oftung, Eva Borka and Abu Salim Mustafa

in FEMS Immunology & Medical Microbiology

Volume 20, issue 4, pages 319-325
Published in print April 1998 | ISSN: 0928-8244
Published online January 2006 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1111/j.1574-695X.1998.tb01142.x
Mycobacterium tuberculosis reactive T cell clones from naturally converted PPD-positive healthy subjects: recognition of the M. tuberculosis 16-kDa antigen

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Abstract

Mycobacterium tuberculosis reactive T cell clones were established from naturally converted PPD-positive healthy subjects and screened for proliferative reactivity against defined M. tuberculosis protein antigens of 16, 19, 65 (HSP65), and 71 (HSP70) kDa recombinantly expressed in Escherichia coli. Among the recombinant antigens tested, the M. tuberculosis 16-kDa protein antigen, as expressed from the λgt11 phage Y3155, was found to induce T cell proliferation. Crossreactivity studies showed that the epitope recognized was present in M. tuberculosis, M. africanum as well as the vaccine strain M. bovis BCG. The M. tuberculosis 16-kDa reactive T cell clone identified showed the CD4+, CD8 phenotype, secreted interferon-γ upon antigen stimulation, and displayed major histocompatibility complex class II restricted cytotoxicity against M. tuberculosis pulsed macrophages. The results obtained suggest that the recombinant M. tuberculosis 16-kDa antigen can be recognized by human Th1 cells with potential relevance to protection.

Keywords: Mycobacterium tuberculosis; 16-kDa antigen; T cell response

Journal Article.  3125 words.  Illustrated.

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