Journal Article

Cytokine secretion profile of human keratinocytes exposed to <i>Malassezia</i> yeasts

Yoshio Ishibashi, Takashi Sugita and Akemi Nishikawa

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 48, issue 3, pages 400-409
Published in print December 2006 | ISSN: 0928-8244
Published online October 2006 | e-ISSN: 1574-695X | DOI: http://dx.doi.org/10.1111/j.1574-695X.2006.00163.x
Cytokine secretion profile of human keratinocytes exposed to Malassezia yeasts

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  • Medical Microbiology and Virology
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Abstract

The lipophilic yeast Malassezia is an exacerbating factor in atopic dermatitis (AD). Among organisms of the Malassezia species, Malassezia globosa and Malassezia restricta are particularly dominant on the skin of AD patients. However, the precise role of Malassezia yeasts in the pathophysiology of AD remains uncertain. Keratinocytes play a critical role in cutaneous inflammatory and immune responses by secreting cytokines. In this study, we attempted to determine the cytokine secretion profiles of human keratinocytes that were exposed to Malassezia yeasts. The human keratinocyte cell line PHK16-0b was cocultivated with M. globosa or M. restricta for 24 h, and the resulting cytokine secretion profile was analysed using a cytokine antibody array. The keratinocytes responded to the two Malassezia species with different Th2-type cytokine profiles, i.e. M. globosa induced IL-5, IL-10 and IL-13 secretion from the keratinocytes, whereas M. restricta induced IL-4 secretion. Similar results were obtained with primary normal human epidermal keratinocytes. cDNA microarray analysis confirmed that IL-5, IL-10, and IL-13 mRNAs were induced only by M. globosa, while IL-4 mRNA expression was induced only by M. restricta. These findings suggest that M. globosa and M. restricta play a synergistic role in triggering or exacerbating AD by stimulating the Th2 immune response.

Keywords: Malassezia; keratinocyte; atopic dermatitis; cytokine

Journal Article.  5614 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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