Journal Article

Identification of putative zinc hydrolase genes of the metallo-β-lactamase superfamily from <i>Campylobacter jejuni</i>

David A. Alfredson and Victoria Korolik

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 49, issue 1, pages 159-164
Published in print February 2007 | ISSN: 0928-8244
Published online January 2007 | e-ISSN: 1574-695X | DOI: http://dx.doi.org/10.1111/j.1574-695X.2006.00197.x
Identification of putative zinc hydrolase genes of the metallo-β-lactamase superfamily from Campylobacter jejuni

More Like This

Show all results sharing these subjects:

  • Medical Microbiology and Virology
  • Biotechnology
  • Genetics and Genomics
  • Microbiology
  • Molecular and Cell Biology

GO

Show Summary Details

Preview

Abstract

DNA fragments encoding two putative zinc-dependent hydrolases, designated GLX2-1 and GLX2-2, from a clinical isolate of Campylobacter jejuni, strain 012, were cloned and sequenced. GLX2-1 was encoded by a sequence of 798 bp and GLX2-2 by a sequence of 597 bp. The amino acid sequences deduced from C. jejuni DNA showed 99% and 100% identity, respectively, to putative zinc hydrolases reported from C. jejuni ATCC strain 11168, and also shared identity (28–43%) with several hypothetical conserved proteins and known zinc-dependent hydrolases and metallo-β-lactamase superfamily proteins. A strictly conserved motif, -H-X-H-X-D-, characteristic of the metallo-β-lactamase superfamily of proteins, including class B metallo-β-lactamases, was identified in both proteins. Other conserved metal-binding ligands, characteristic of the metallo-β-lactamase superfamily of proteins, were also identified. Functional β-lactamase could not be expressed in either Escherichia coli or Campylobacter coli transformed with C. jejuni hydrolase-containing plasmids, suggesting that they do not function as metallo-β-lactamases, although structurally they are consistent with the zinc metallo-hydrolase family of the β-lactamase fold.

Keywords: Campylobacter; metallo-β-lactamase; zinc hydrolase

Journal Article.  3596 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.