Journal Article

<i>Astragalus</i> polysaccharides enhance the humoral and cellular immune responses of hepatitis B surface antigen vaccination through inhibiting the expression of transforming growth factor β and the frequency of regulatory T cells

Xiaogang Du, Xiaobing Chen, Bing Zhao, Yao Lv, Huaiyu Zhang, Hanmei Liu, Zhiyu Chen, Yanger Chen and Xianyin Zeng

in FEMS Immunology & Medical Microbiology

Volume 63, issue 2, pages 228-235
Published in print November 2011 | ISSN: 0928-8244
Published online October 2011 | e-ISSN: 1574-695X | DOI: http://dx.doi.org/10.1111/j.1574-695X.2011.00845.x
Astragalus polysaccharides enhance the humoral and cellular immune responses of hepatitis B surface antigen vaccination through inhibiting the expression of transforming growth factor β and the frequency of regulatory T cells

Show Summary Details

Preview

Abstract

Astragalus polysaccharides (APS), extracted from the root of Astragalus membranaceus, a traditional Chinese medicinal herb, have extensive pharmacological and strong immunomodulatory effects. In this study, the potential adjuvant effect of APS on humoral and cellular immune responses to hepatitis B subunit vaccine was investigated. Coadministration of APS with recombinant hepatitis B surface antigen significantly increased antigen-specific antibody production, T-cell proliferation and CTL (cytotoxic T lymphocyte) activity. Production of interferon-γ (IFN-γ), interleukin-2 (IL-2) and IL-4 in CD4+ T cells and of IFN-γ in CD8+ T cells were dramatically increased. Furthermore, expression of the genes PFP, GraB, Fas L and Fas were up-regulated; interestingly, expression of transforming growth factor β (TGF-β) and the frequency of CD4+CD25+Foxp3+ regulatory T cells (Treg cells) were down-regulated. Expression of Toll-like receptor 4 (TLR4) was significantly increased by administration of APS. Together, these results suggest that APS is a potent adjuvant for the hepatitis B subunit vaccine and can enhance both humoral and cellular immune responses via activating the TLR4 signaling pathway and inhibit the expression of TGF-β and frequency of Treg cells.

Keywords: Astragalus polysaccharides; hepatitis B vaccine; adjuvant; immune response; TLR-4; TGF-β; Treg

Journal Article.  3440 words.  Illustrated.

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.