Journal Article

Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, <i>Methylomonas aminofaciens</i> 77a, and its expression in <i>Escherichia coli</i>

Hideshi Yanase, Kohei Ikeyama, Ryoji Mitsui, Sonmin Ra, Keiko Kita, Yasuyoshi Sakai and Nobuo Kato

in FEMS Microbiology Letters

Volume 135, issue 2-3, pages 201-205
Published in print January 1996 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb07990.x

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Abstract

A DNA fragment of 550 bp was specifically amplified by PCR with primers based on the N-terminal sequence of the purified 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a and on that of a lysyl endopep(idase-derived peptide. Using this PCR product as a probe, a gene coding for 3-hexulose-6-phosphate synthase in M. aminofaciens 77a chromosomal DNA was cloned in Escherichia coli JM109. Sequencing analysis revealed that the gene encoding 3-hexulose-6-phosphate synthase contained a 624-bp open reading frame, encoding a protein composed of 208 amino acid residues with a calculated relative molecular mass of 21 224.

Keywords: Hexulose phosphate synthase gene; Methylotroph; Ribulose monophosphate cycle; Methylomonas aminofaciens

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