Journal Article

Mutations in <i>Bacillus subtilis</i> PyrR, the <i>pyr</i> regulatory protein, with defects in regulation by pyrimidines

Sa-Youl Ghim and Robert L. Switzer

in FEMS Microbiology Letters

Volume 137, issue 1, pages 13-18
Published in print March 1996 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08075.x

Show Summary Details

Preview

Abstract

The pyrimidine nucleotide biosynthetic (pyr) operon in Bacillus subtilis is regulated by a transcriptional attenuation mechanism in which PyrR, a bifunctional pyr RNA-binding attenuation protein/uracil phosphoribosyltransferase, plays a crucial role. A convenient procedure for isolation of pyrR mutants with defects in the regulation of pyr operon expression is described. The selection is based on the selection of spontaneous mutations that convert the pyrimidine-sensitive growth of cpa strain (lacking arginine-repressible carbamyl phosphate synthetase) to pyrimidine resistance. Twelve such mutants were isolated and sequenced. All resulted from point mutations in the pyrR gene.

Keywords: Bacillus subtilis; Mutations; Pyrimidine biosynthesis; PyrR; Regulatory protein; Transcriptional attenuation

Journal Article.  0 words. 

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.