Journal Article

Pulsed-field gel electrophoresis indicates genotypic heterogeneity among <i>Campylobacter upsaliensis</i> strains

Billy Bourke, Philip M. Sherman, David Woodward, Hermy Lior and Voon L. Chan

in FEMS Microbiology Letters

Volume 143, issue 1, pages 57-61
Published in print September 1996 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08461.x

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Abstract

To determine the genome relatedness among a selection of animal and human Campylobacter upsaliensis isolates, macrorestriction profiles were generated for 20 C. upsaliensis strains, among 7 serogroups, using pulsed-field gel electrophoresis (PFGE). XhoI, SalI and SacII restriction enzyme profiles indicated genomic heterogeneity among strains. Using XhoI and SacII restriction enzyme digestion, genomic similarities between some pairs of strains were Lior serogroup specific. The genomic sizes of these isolates varied from 1.74 to 2.09 Mb. These results demonstrate molecular heterogeneity of this species similar to that found among Helicobacter pylori isolates. Among C. upsaliensis strains, PFGE is highly discriminatory and should prove a useful molecular typing method for epidemiological purposes.

Keywords: Campylobacter; Epidemiology; Chromosome

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