Journal Article

Kinetic and antigenic similarities for cellobiose dehydrogenase from the brown rot fungus <i>Coniophora puteana</i> and the white rot fungus <i>Phanerochaete chrysosporium</i>

Simon M. Hyde and Paul M. Wood

in FEMS Microbiology Letters

Volume 145, issue 3, pages 439-444
Published in print December 1996 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.1996.tb08613.x

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Abstract

Cellobiose dehydrogenase was purified from the brown rot fungus Coniophora puteana. Strong cross-reaction was observed with antibodies to cellobiose:quinone oxidoreductase from the white rot fungus Phanerochaete chrysosporium. Kinetic measurements were made with cellobiose as electron donor. Ferricyanide and DCPIP both showed a pH optimum close to pH 4, but activity with ferricyanide declined more rapidly when the pH was raised. Dioxygen reduction to hydrogen peroxide was observed, but at a much lower rate than for other acceptors. These properties are similar to those of cellobiose dehydrogenase from P. chrysosporium, despite differences between brown and white rot modes of decay.

Keywords: Cellobiose dehydrogenase; Coniophora puteana; Lignocellulose breakdown; Phanerochaete chrysosporium

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