Journal Article

Analysis of plasmid profiles of <i>Aeromonas salmonicida</i> isolates by pulsed field gel electrophoresis

M.Anne Livesley, Stephen N Smith, Richard A Armstrong and Gavin A Barker

in FEMS Microbiology Letters

Volume 146, issue 2, pages 297-301
Published in print January 1997 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.1997.tb10208.x

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Abstract

Plasmid constitutions of Aeromonas salmonicida isolates were characterised by flat-bed and pulsed field gel electrophoresis. Resolution of plasmids by pulsed field gel electrophoresis was greater and more consistent than that achieved by flat-bed gel electrophoresis. The number of plasmids separated by pulsed field gel electrophoresis varied between A. salmonicida isolates, with five being the most common number present in the isolates used in this study. Plasmid profiles were diverse and the reproducibility of the distances migrated facilitated the use of principal components analysis for the characterisation of the isolates. Isolates were grouped according to the number of plasmids supported. Further principal components analysis of groups of isolates supporting five and seven plasmids showed a spatial separation of plasmids based upon distance migrated. Principal components analysis of plasmid profiles and antimicrobial minimum inhibitory concentrations could not be correlated suggesting that resistance to antimicrobial agents is not associated with either one plasmid or a particular plasmid constitution.

Keywords: Aeromonas salmonicida; Pulsed field gel electrophoresis; Principal components analysis; Plasmid

Journal Article.  1959 words.  Illustrated.

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