Journal Article

Expression of the <i>tetA</i>(C) tetracycline efflux pump in <i>Escherichia coli</i> confers osmotic sensitivity

Tom A. Stavropoulos and Craig A. Strathdee

in FEMS Microbiology Letters

Volume 190, issue 1, pages 147-150
Published in print September 2000 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.2000.tb09277.x

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Expression of tetA(C) in Escherichia coli confers resistance to tetracycline as well as sensitivity to nickel and cadmium salts, lipophilic chelating agents, and aminoglycoside antibiotics. In this report we determine that high-level expression of tetA(C) also confers an osmotic sensitivity. The osmotic-sensitive phenotype is distinct from the tetracycline-resistant phenotype and can be localized to a domain contained within the first 98 amino acid residues of the TetA(C) polypeptide.

Keywords: Tetracycline antiporter; Tetracycline resistance; Membrane integrity; Nichel sensitivity; Osmotic sensitivity; Escherichia coli

Journal Article.  1655 words.  Illustrated.

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