Journal Article

Combined use of growth rate correlated and growth rate independent promoters for recombinant glucoamylase production in <i>Fusarium venenatum</i>

Caroline Gordon, Sian Thomas, Alison Griffen, Geoffrey D. Robson, Anthony P.J. Trinci and Marilyn G. Wiebe

in FEMS Microbiology Letters

Volume 194, issue 2, pages 229-234
Published in print January 2001 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: https://dx.doi.org/10.1111/j.1574-6968.2001.tb09474.x

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Abstract

Fusarium venenatum JeRS 325, a strain which produces recombinant glucoamylase under control of a growth rate independent promoter was transformed with a plasmid carrying the Aspergillus niger glucoamylase gene under control of its own growth rate correlated promoter. Some disruption of the original recombinant genes occurred and at pH 5.8 the double transformant did not produce as much glucoamylase as JeRS 325 in batch culture. However, the double transformant still produced as much glucoamylase as JeRS 325 in fed-batch cultures, illustrating the potential for the combined use of growth rate independent and dependent promoters to improve production of recombinant proteins in fed-batch culture systems.

Keywords: Glucoamylase; Recombinant protein; Promoter; Fusarium venenatum

Journal Article.  3258 words.  Illustrated.

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