Journal Article

Assessment of the serodiagnostic potential of nine novel proteins from <i>Mycobacterium tuberculosis</i>

Alison J. Moran, Janice D. Treit, Janice L. Whitney, Bassam Abomoelak, Raymond Houghton, Yasir A.W. Skeiky, Diana Pedral Sampaio, Roberto Badaró and Francis E. Nano

in FEMS Microbiology Letters

Volume 198, issue 1, pages 31-36
Published in print April 2001 |
Published online January 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.2001.tb10615.x

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Abstract

To identify antigens that would improve the accuracy of serological diagnosis of active tuberculosis, we cloned the genes encoding nine potentially immunogenic secreted or surface-associated proteins of Mycobacterium tuberculosis. Recombinant proteins were reacted with sera from HIV-negative individuals with extrapulmonary tuberculosis (EP-TB) or HIV-positive individuals with pulmonary tuberculosis (TBH). Specific and high level antibody responses were obtained for four recombinant proteins, of which antigen GST-822 was recognized by 60% of EP-TB and 42% of TBH and antigen MBP-506 was recognized by 45% of EP-TB and 61% of TBH. These results suggest that these proteins are strong candidates as subunits in a polyvalent serodiagnostic test.

Keywords: Serodiagnosis; Tuberculosis; Antigen

Journal Article.  2902 words.  Illustrated.

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