Journal Article

Characterization of the <i>Legionella anisa</i> population structure by pulsed-field gel electrophoresis

Mongi Akermi, Anne Doleans, Françoise Forey, Monique Reyrolle, Helene Meugnier, Jean Freney, François Vandenesch, Jerome Etienne and Sophie Jarraud

in FEMS Microbiology Letters

Volume 258, issue 2, pages 204-207
Published in print May 2006 |
Published online March 2006 | e-ISSN: 1574-6968 | DOI: https://dx.doi.org/10.1111/j.1574-6968.2006.00216.x

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Abstract

We analysed 38 French isolates of Legionella anisa by means of pulsed-field gel electrophoresis (PFGE) with single or double digestion. Double digestion was more discriminatory than single digestion, and can thus be useful for epidemiological studies of L. anisa. Several isolates from different parts of France clustered together on the basis of their PFGE patterns (similarity cutoff of 80%), suggesting that the L. anisa population structure is homogenous or that a few clones of L. anisa strains have spread widely in France.

Keywords: Legionella anisa; pulsed field gel electrophoresis; population structure

Journal Article.  1688 words.  Illustrated.

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