Journal Article

Effect of the morphogene <i>bolA</i> on the permeability of the <i>Escherichia coli</i> outer membrane

Patrick Freire, Helena L.A. Vieira, Ana R. Furtado, Miguel A. De Pedro and Cecília M. Arraiano

in FEMS Microbiology Letters

Volume 260, issue 1, pages 106-111
Published in print July 2006 |
Published online April 2006 | e-ISSN: 1574-6968 | DOI: http://dx.doi.org/10.1111/j.1574-6968.2006.00307.x

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Abstract

Escherichia coli bolA is a morphogene involved in stress response and cell division. Overexpression of bolA induces biofilm formation and affects the levels of carboxypeptidases PBP5, PBP6 and β-lactamase AmpC. In this study, we have shown that changes in the expression of bolA result in alterations in the properties of the outer membrane. The sensitivity to detergents and vancomycin was reduced when bolA was overexpressed and fluorescent probes indicated that different levels of bolA had an effect on outer membrane protein accessibility. Moreover, bolA was shown to be involved in the modulation of the OmpF/OmpC balance.

Keywords: bolA; permeability; Escherichia coli; outer membrane; cell morphology

Journal Article.  3548 words.  Illustrated.

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