Journal Article

Specific expression of short-interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells

Xuejing Luan, Limin Guo, Zuozhen Yang, Min Liu, Xin Li and Hua Tang

in Acta Biochimica et Biophysica Sinica

Published on behalf of Institute of Biochemistry and Cell Biology, SIBS, CAS

Volume 40, issue 11, pages 928-933
Published in print November 2008 | ISSN: 1672-9145
Published online November 2008 | e-ISSN: 1745-7270 | DOI: https://dx.doi.org/10.1111/j.1745-7270.2008.00475.x
Specific expression of short-interfering RNA driven by human telomerase reverse transcriptase promoter in tumor cells

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RNA interference (RNAi) has been shown to be an effective method for inhibiting the expression of a given gene in human cells by targeting with short duplex RNA (short-interfering RNA or siRNA). However, more and more studies suggest that non-specific effects can be induced by siRNAs, such as off-target inhibition, activation of interferon response, and saturation of cellular silencing machinery. It has been known that more than 90% of human tumors exhibit telomerase activity. Consequently, telomerase is believed to be a broadspectrum molecular marker of malignancies. In the present study we attempt to develop a tumor-specific RNAi system using the human telomerase reverse transcriptase promoter. This system may provide a basis for RNAi therapy.

Keywords: RNAi; short-interfering RNA; telomerase; hTERT promoter; gene therapy

Journal Article.  0 words. 

Subjects: Biochemistry

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