Journal Article

Are Fas Ligand Polymorphisms Associated With Occult HBV Infection?

Mohammad Kazemi Arababadi, Adel Mohammadzadeh, Behzad Nasiri Ahmadabadi, Ali Akbar Pourfathollah and Derek Kennedy

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 41, issue 11, pages 672-675
Published in print November 2010 | ISSN: 0007-5027
Published online October 2015 | e-ISSN: 1943-7730 | DOI: http://dx.doi.org/10.1309/LM7CH48RQRLRDLDZ
Are Fas Ligand Polymorphisms Associated With Occult HBV Infection?

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Background:

Occult hepatitis B infection (OBI) is a form of hepatitis in which there is an absence of detectable HBsAg, despite the presence of HBV-DNA in the peripheral blood of patients. The main aim of this study was to investigate the relationship between polymorphisms in the -844 and IVS2nt-124 regions of the Fas ligand (FasL) gene with OBI.

Materials and Methods:

The plasma samples from 3700 blood donors were tested for HBsAg and anti-HBs by enzyme-linked immunosorbent assay (ELISA). The HBsAg/anti-HBc+ samples were selected and screened for HBV-DNA by polymerase chain reaction (PCR). HBV-DNA positive samples were assigned as OBI cases, and PCR-RFLP techniques were performed to examine the polymorphisms.

Results:

Of 3700 blood samples, 352 (9.5%) were HBsAg/anti-HBc+, and HBV-DNA was detected in 57/352 (16.1%) of HBsAg/anti-HBc+ samples and designated as OBI patients. Our results showed the patient and control groups had no significant differences regarding the studied polymorphisms.

Conclusion:

Based on our results, it can be concluded that the functional polymorphisms in the promoter region of the FasL gene are not associated with OBI.

Keywords: occult hepatitis B infection; FasL; polymorphism; HBsAg; HBV-DNA

Journal Article.  3041 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Microbiology

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