Journal Article

The Association of NAD(P)H:quinine Oxidoreductase Gene Polymorphisms With Pediatric Acute Lymphoblastic Leukemia

Farhad Zaker, Akram Safaei, Nahid Nasiri, Maryam Abdollahzadeh and Vahid Pazhakh

in Laboratory Medicine

Published on behalf of American Society for Clinical Pathology

Volume 43, issue 6, pages 256-261
Published in print November 2012 | ISSN: 0007-5027
Published online September 2015 | e-ISSN: 1943-7730 | DOI: http://dx.doi.org/10.1309/LMTFQ7ZO8YEGAJ1H
The Association of NAD(P)H:quinine Oxidoreductase Gene Polymorphisms With Pediatric Acute Lymphoblastic Leukemia

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  • Haematology
  • Clinical Cytogenetics and Molecular Genetics
  • Molecular and Cell Biology
  • Molecular Biology and Genetics

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Objectives

Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer. NAD(P)H:quinone oxidoreductase 1 (NQO1) is an enzyme that protects cells against mutagenicity of free radicals and toxic oxygen metabolites. C to T base substitution at nucleotides 609 and 465 of NQO1 cDNA, results in loss of enzyme activity. Low NQO1 activity may play a role in etiology of ALL. In the present study, we investigated the association between the NQO1 polymorphisms and increased risk of ALL in children.

Methods

C609T and C465T polymorphisms of NQO1 were explored using polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) assay in 100 pediatric ALL patients and 135 healthy controls.

Results

Although C609T polymorphism is very common among the Iranian population, we found no association between this variant and increased risk for pediatric ALL [odds ratio (OR) = 0.95; 95% confidence interval (95% CI) = 0.55–1.64]. Interestingly the other polymorphic allele of NQO1 (C465T) was strongly associated with pediatric ALL (OR = 7.83; 95% CI= 3.27-18.75).

Conclusion

These findings do not support the predisposing role of NQO1 C609T polymorphism for pediatric ALL. However, The C465T polymorphism was associated with increased risk of pediatric ALL. Further studies with larger sample including evaluating multiple gene–gene interactions seem necessary to validate the exact role of these mutations.

Keywords: Acute Lymphoblastic Leukemia; polymorphism; NQO1; RFLP

Journal Article.  3188 words.  Illustrated.

Subjects: Haematology ; Clinical Cytogenetics and Molecular Genetics ; Molecular and Cell Biology ; Molecular Biology and Genetics

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