Materials and Methods

Yuri. L. R. Leite

in Evolution and Systematics of the Atlantic Tree Rats, Genus Phyllomys (Rodentia, Echimyidae), With Description of Two New Species

Published by University of California Press

Published in print October 2003 | ISBN: 9780520098497
Published online March 2012 | e-ISBN: 9780520916074 | DOI:
Materials and Methods

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Specimens of Phyllomys consist of skins, skulls, post-cranial skeletons, and fluid-preserved material housed. Specimens used in the molecular analyses were collected in the field during several expeditions led by different research groups. Specimens were collected using Sherman, Tomahawk, or locally made live traps placed either on the ground or in the tree branches and vines. Some were collected during night censuses, using a .410-gauge shotgun loaded with dust shot (#12), and others were caught by hand from nests in tree hollows during the day. Voucher specimens were preserved as study skins and skulls or in fluid, following standard museum procedures. Liver samples were preserved in ethyl alcohol and liquid nitrogen from DNA analyses. Two-way analyses of variance (ANOVA) were used to examine non-geographic variation (due to sex and age). Principal components analyses (PCA) were used to analyze how individual measurements contribute to the total pool of variation.

Keywords: skeletons; Sherman; Tomahawk; specimens; ANOVA

Chapter.  3468 words.  Illustrated.

Subjects: Zoology and Animal Sciences

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