Chapter

Immunophenotyping of Avian Lymphocytes

Jeanne M. Fair, Kirsten J. Taylor-McCabe, Yulin Shou and Babetta L. Marrone

in Emerging Avian Disease

Published by University of California Press

Published in print April 2012 | ISBN: 9780520272378
Published online September 2012 | e-ISBN: 9780520952201 | DOI: http://dx.doi.org/10.1525/california/9780520272378.003.0007
Immunophenotyping of Avian Lymphocytes

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Chicken (Gallus gallus domesticus)T-cell populations can be delineated into subsets based on their expression of cell-surface proteins such as cluster of differentiation (CD) cell surface markers. However, immunophenotyping using flow cytometry in birds has focused on cell characterization in the thymus and spleen during development in chickens. West Nile virus (WNV) causes differential infections in birds, ranging the entire spectrum of pathogenesis. In order to accurately assess immunocompetence to diseases such as WNV in birds, more efficient methodology to access natural variability in avian immune function must be devised and understood. Previously, lymphocyte subpopulations CD4+ and CD8+ have been found to be critical for clearing infection of WNV in mammals. Focusing on chickens, a species that is susceptible but not infective for WNV, our objectives were to: (1) further develop flow cytometry for estimating subpopulations of lymphocytes in peripheral blood from poultry, (2) estimate the best antibody and cell marker combination for estimating lymphocyte subpopulations, and (3) estimate repeatability and application to other avian species susceptible to WNV. Immunophenotyping of CD3+, CD4+, CD8+, and CD45+ was successfully completed for chicken peripheral blood but not for the Common Raven (Corvus corax) or Black-billed Magpie (Pica hudsonia). Future studies include immunophenotyping during infection studies of WNV in chickens and further development of flow cytometry for other bird species.

Keywords: chicken; flow cytometry; Gallus gallus domesticus; host range; immunophenotyping, lymphocytes; West Nile virus

Chapter.  6013 words.  Illustrated.

Subjects: Vertebrates

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