Journal Article

An Insight Into the Sialotranscriptome of <i>Triatoma rubida</i> (Hemiptera: Heteroptera)

in Journal of Medical Entomology

Published on behalf of Entomological Society of America

Volume 49, issue 3, pages 563-572
Published in print May 2012 | ISSN: 0022-2585
Published online November 2014 | e-ISSN: 1938-2928 | DOI: http://dx.doi.org/10.1603/ME11243
An Insight Into the Sialotranscriptome of Triatoma rubida (Hemiptera: Heteroptera)

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The kissing bug Triatoma rubida (Uhler, 1894) is found in southwestern United States and parts of Mexico where it is found infected with Trypanosoma cruzi, invades human dwellings and causes allergies from their bites. Although the protein salivary composition of several triatomine species is known, not a single salivary protein sequence is known from T. rubida. Furthermore, the salivary diversity of related hematophagous arthropods is very large probably because of the immune pressure from their hosts. Here we report the sialotranscriptome analysis of T. rubida based on the assembly of 1,820 high-quality expressed sequence tags, 51% of which code for putative secreted peptides, including lipocalins, members of the antigen five family, apyrase, hemolysin, and trialysin families. Interestingly, T. rubida lipocalins are at best 40% identical in primary sequence to those of T. protracta, a kissing bug that overlaps its range with T. rubida, indicating the diversity of the salivary lipocalins among species of the same hematophagous genus. We additionally found several expressed sequence tags coding for proteins of clear Trypanosoma spp. origin. This work contributes to the future development of markers of human and pet exposure to T. rubida and to the possible development of desensitization therapies. Supp. Data 1 and 2(online only) of the transcriptome and deducted protein sequences can be obtained from http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S1-web.xlsx and http://exon.niaid.nih.gov/transcriptome/Trubida/Triru-S2-web.xlsx.

Keywords: Chagas disease; vector biology; salivary gland; sialome; transcriptome

Journal Article.  7401 words.  Illustrated.

Subjects: Disease Ecology and Epidemiology ; Entomology

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