Journal Article

Absence of functional TLR4 impairs response of macrophages after <i>Candida albicans</i> infection

Thaís Helena Gasparoto, Vanessa Tessarolli, Thiago Pompermaier Garlet, Sérgio Aparecido Torres, Gustavo Pompermaier Garlet, João Santana Da Silva and Ana Paula Campanelli

in Medical Mycology

Published on behalf of International Society for Human and Animal Mycology

Volume 48, issue 8, pages 1009-1017
Published in print December 2010 | ISSN: 1369-3786
Published online December 2010 | e-ISSN: 1460-2709 | DOI: http://dx.doi.org/10.3109/13693786.2010.481292
Absence of functional TLR4 impairs response of macrophages after Candida albicans infection

More Like This

Show all results sharing these subjects:

  • Mycology and Fungi
  • Infectious Diseases
  • Medical Toxicology
  • Veterinary Medicine
  • Environmental Science

GO

Show Summary Details

Preview

Candida albicans is recognized by phagocytic cells through a set of recognition receptors patterns. Recently, we showed the importance of TLR2 in the regulation of neutrophil survival after C. albicans infection. In the present work, we analyzed the involvement of TLR4 in the recognition of C. albicans by neutrophils and macrophages. Our results show that the absence of functional TLR4 resulted in lower chemotaxis of neutrophils to the site of infection, lower levels of TNF-α, CXCL1 and nitric oxide, and dissemination and persistence of the pathogen in lymph nodes and spleen. In vitro, the phagocytic activity, nitric oxide production and myeloperoxidase activity, CXCL1, IL-1β production by neutrophils from TLR4-defective mice were not changed. In contrast, macrophages from TLR4-defective mice demonstrated lower phagocytosis and lower levels of CXCL1, IL-1β and TNF-α. Together, these data demonstrate that TLR4 signals are important for the recognition of C. albicans by macrophages and their absence allows persistence of the infection.

Keywords: TLR4; innate response; Candida albicans

Journal Article.  4194 words.  Illustrated.

Subjects: Mycology and Fungi ; Infectious Diseases ; Medical Toxicology ; Veterinary Medicine ; Environmental Science

Full text: subscription required

How to subscribe Recommend to my Librarian

Users without a subscription are not able to see the full content. Please, subscribe or login to access all content.