Journal Article

Characterization of the antimicrobial susceptibility of fungi responsible for onychomycosis in Spain

A. Zalacain, C. Obrador, J. P. Martinez, M. Viñas and T. Vinuesa

in Medical Mycology

Published on behalf of International Society for Human and Animal Mycology

Volume 49, issue 5, pages 495-499
Published in print July 2011 | ISSN: 1369-3786
Published online July 2011 | e-ISSN: 1460-2709 | DOI: http://dx.doi.org/10.3109/13693786.2010.541949
Characterization of the antimicrobial susceptibility of fungi responsible for onychomycosis in Spain

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  • Mycology and Fungi
  • Infectious Diseases
  • Medical Toxicology
  • Veterinary Medicine
  • Environmental Science

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Due to the increase of choices relative to antifungals, there is a need to improve the standardization of in vitro methods used to determine the antifungal susceptibility of fungal pathogens. Our study evaluated the in vitro susceptibility of filamentous fungi isolated from patients with toenail onychomycosis against itraconazole, ciclopirox, eberconazole, fluconazole and terbinafine. The minimum inhibitory concentration (MIC) of these antifungal agents was determined with 100 isolates, including dermatophytes (70 strains) and non-dermatophyte molds (30 strains). The susceptibility of fungal isolates was measured by using a technique modified for dermatophytes (0.5 × 103–0.5 × 104 conidia/ml as inocula) which followed the procedures described by the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST) and the Clinical and Laboratory Standard Institute (CLSI M38-A). MIC ranges were 0.016–8.0 µg/ml for itraconazole, ciclopirox and eberconazole, 0.063–32.0 µg/ml for fluonazole, and 0.004–2.0 µg/ml for terbinafine. In vitro susceptibility tests indicated that eberconazole has a broad antimicrobial profile, including dermatophytes, as well as other filamentous fungi. Terbinafine was active (0.016–0.250 µg/ml) against dermatophytes.

Keywords: antifungal agents; in vitro susceptibility testing; onychomycosis

Journal Article.  2359 words. 

Subjects: Mycology and Fungi ; Infectious Diseases ; Medical Toxicology ; Veterinary Medicine ; Environmental Science

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