Journal Article

Effects of cold stress on the messenger ribonucleic acid levels of peroxisome proliferator-activated receptor-γ in spleen, thymus, and bursa of Fabricius of chickens

J. T. Wang, S. Li, J. L. Li, J. W. Zhang and S. W. Xu

in Poultry Science

Published on behalf of Poultry Science Association Inc.

Volume 88, issue 12, pages 2549-2554
Published in print December 2009 | ISSN: 0032-5791
Published online December 2009 | e-ISSN: 1525-3171 | DOI: https://dx.doi.org/10.3382/ps.2009-00404
Effects of cold stress on the messenger ribonucleic acid levels of peroxisome proliferator-activated receptor-γ in spleen, thymus, and bursa of Fabricius of chickens

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ABSTRACT

This study was to investigate the expression trait of the peroxisome proliferator-activated receptor-γ (PPAR-γ) gene and the effect of cold stress on the mRNA levels of PPAR-γ in spleen, thymus, and bursa of Fabricius of chickens. Eighty-four 1-d-old male chickens were randomly allocated to 12 groups (7 chickens per group). There was 1 control group and 5 treatment groups for acute cold stress and 3 control groups and 3 treatment groups for chronic cold stress. Chickens were maintained in our animal facility, kept under a 16L:8D cycle and temperature (30 ± 2°C), and given free access to standard chow and water. The cold stress was initiated when the birds were 15 d of age, with the duration of the acute cold stress being 1, 3, 6, 12, and 24 h, and the chronic cold stress was 5, 10, and 20 d, respectively. Cold stress temperature was 12 ± 1°C. Spleen, thymus, and bursa of Fabricius were collected for the assessment of the mRNA levels by real-time PCR after stress termination. The results showed that the PPAR-γ gene is expressed in spleen, thymus, and bursa of Fabricius, and its expression level is different in different tissues and at different ages. Acute cold stress significantly decreased (P < 0.05) the mRNA levels of the PPAR-γ gene of spleen and thymus in all treatment groups and significantly increased (P < 0.05) the mRNA levels of the PPAR-γ gene of bursa of Fabricius in all treatment groups. Compared with the corresponding control groups, chronic cold stress resulted in a significant increase (P < 0.05) of the mRNA levels of the PPAR-γ gene in spleen and a significant decrease (P < 0.05) of the mRNA levels of the PPAR-γ gene in thymus and bursa of Fabricius. The results indicate that the PPAR-γ gene is expressed in all 3 immune organs and has different expression traits. The magnitude and direction of change in PPAR-γ gene expression differs with the type of cold stress applied and also varies by tissue.

Keywords: spleen; thymus; bursa of Fabricius; cold stress; peroxisome proliferator-activated receptor-γ

Journal Article.  3204 words.  Illustrated.

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