Journal Article

Effects of zearalenone on calcium homeostasis of splenic lymphocytes of chickens in vitro

Y. C. Wang, J. L. Deng, S. W. Xu, X. Peng, Z. C. Zuo, H. M. Cui, Y. Wang and Z. H. Ren

in Poultry Science

Published on behalf of Poultry Science Association Inc.

Volume 91, issue 8, pages 1956-1963
Published in print August 2012 | ISSN: 0032-5791
Published online August 2012 | e-ISSN: 1525-3171 | DOI: https://dx.doi.org/10.3382/ps.2011-02128
Effects of zearalenone on calcium homeostasis of splenic lymphocytes of chickens in vitro

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ABSTRACT

Zearalenone (ZEA) is an estrogenic mycotoxin. It is produced by several Fusarium species and can contaminate food and feed. To investigate the role of calcium homeostasis in ZEA-induced toxicity of poultry and elucidate its cytotoxic mechanism, splenic lymphocytes isolated from chickens were exposed to ZEA (0–25 μg/mL) for 48 h. The intracellular calcium concentration ([Ca2+]i), pH, calmodulin (CaM) mRNA levels, and Na+/K+-ATPase activities and Ca2+-ATPase activities were detected by the fluorescent dyes Fluo-3/AM and BCECF/AM, quantitative real-time PCR, and chromatometry. Supernatant CaM concentrations were simultaneously detected by ELISA. As the ZEA exposure concentration increased, the [Ca2+]i and CaM mRNA levels gradually increased, while intracellular pH, CaM concentrations of supernatants, and intracellular Na+,K+-ATPase and Ca2+-ATPase activities gradually decreased in a dose-dependent manner. There were significant differences (P < 0.05 or P < 0.01) between the treatment groups and the control group. These results indicate that ZEA cytotoxicity arises by causing an imbalance in calcium homeostasis and intracellular acidification in lymphocytes.

Keywords: zearalenone; calcium homeostasis; ATPase; calmodulin; mycotoxin

Journal Article.  4775 words.  Illustrated.

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