Journal Article

PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers

S. Cortes, N. Rolão, J. Ramada and L. Campino

in Transactions of The Royal Society of Tropical Medicine and Hygiene

Published on behalf of Royal Society of Tropical Medicine and Hygiene

Volume 98, issue 1, pages 12-17
Published in print January 2004 | ISSN: 0035-9203
Published online January 2004 | e-ISSN: 1878-3503 | DOI: https://dx.doi.org/10.1016/S0035-9203(03)00002-6
PCR as a rapid and sensitive tool in the diagnosis of human and canine leishmaniasis using Leishmania donovani s.l.-specific kinetoplastid primers

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  • Clinical Medicine
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Abstract

This study was performed in order to test the efficacy of a new polymerase chain reaction (PCR) assay for the diagnosis of both human and canine leishmaniasis caused by Leishmania infantum. The new primers were developed on the basis of a complete DNA sequence of the L. infantum kinetoplast minicircle. Specificity and sensitivity were evaluated by testing bone marrow spots on filter paper and skin biopsy samples, and the PCR results were compared to data from in vitro cultures. Leishmania strains from different foci, as well as other trypanosomatids and opportunistic pathogenic micro-organisms, were also included in this study. The results show that the primers are highly specific, detecting only L. donovani s.l. DNA, and sensitive for the detection of parasite DNA in biological samples from three different geographical regions of Portugal (north, centre and south) and from Brazil.

Keywords: Leishmaniasis; Leishmania donovani s.l.; Polymerase chain reaction; Kinetoplastid primers

Journal Article.  2892 words.  Illustrated.

Subjects: Clinical Medicine ; Infectious Diseases ; Immunology ; Medical Microbiology and Virology ; Public Health and Epidemiology

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