Journal Article

The acute phase protein serum amyloid A primes neutrophils

Elaine Hatanaka, Fernanda Pereira Ribeiro and Ana Campa

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 38, issue 1, pages 81-84
Published in print August 2003 | ISSN: 0928-8244
Published online January 2006 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1016/S0928-8244(03)00112-3
The acute phase protein serum amyloid A primes neutrophils

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  • Medical Microbiology and Virology
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Abstract

We studied here the effect of the acute phase protein serum amyloid A (SAA) on the oxidative burst of neutrophils. Incubation of neutrophils with SAA increased the rate of oxygen uptake and the production of reactive oxygen species of neutrophils activated with opsonized zymosan (OZ). The increment in the neutrophil oxidative burst was dependent on SAA concentration in the range of 3–33 µg protein ml−1 and was observed only in the presence of a relatively low amount of OZ (1×106 particles ml−1). SAA did not affect oxygen consumption and reactive oxygen production triggered by other stimuli, such as f-Met-Leu-Phe, phorbol myristate acetate or non-opsonized zymosan. Our finding points to a priming effect of SAA probably associated with mobilization of receptors for opsonized particles and strengthens the role of SAA as an effector of neutrophil functions in inflammation.

Keywords: Serum amyloid A; Neutrophil; Acute phase protein; Oxidative burst; Luminol-enhanced chemiluminescence; Priming; Reactive oxygen species

Journal Article.  2239 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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