Journal Article

Subtracted restriction fingerprinting — a new typing technique using magnetic capture of tagged restriction fragments

Valeri Terletski, Geovana Brenner Michael and Stefan Schwarz

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 41, issue 1, pages 1-8
Published in print May 2004 | ISSN: 0928-8244
Published online January 2006 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1016/j.femsim.2004.01.009
Subtracted restriction fingerprinting — a new typing technique using magnetic capture of tagged restriction fragments

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Abstract

Molecular typing of bacterial pathogens is an important issue in the epidemiological analysis of emerging infections in humans and animals. Numerous methods have been developed for and applied to a wide variety of bacteria of medical, veterinary and zoonotic importance. The present minireview provides a description of a new typing approach designated subtracted restriction fingerprinting (SRF), its use for typing of Salmonella isolates and a comparison with the most widely used typing techniques for these bacteria. SRF is based on double restriction endonuclease digestion of whole cell DNA, followed by a fill-in reaction with specifically tagged nucleotides and subtractive capture of selected restriction fragments. This results in a reduced number of fragments optimal for separation in standard agarose gels.

Keywords: Salmonella; Typing; Restriction endonuclease analysis; Epidemiology

Journal Article.  5192 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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