Journal Article

Immunization with glutathione S-transferase and mutant toxic shock syndrome toxin 1 fusion protein protects against Staphylococcus aureus infection

Jing-Chun Cui, Dong-Liang Hu, Yan-Chun Lin, Ai-Dong Qian and Akio Nakane

in FEMS Immunology & Medical Microbiology

Published on behalf of Federation of Microbiological Societies

Volume 45, issue 1, pages 45-51
Published in print July 2005 | ISSN: 0928-8244
Published online April 2006 | e-ISSN: 1574-695X | DOI: https://dx.doi.org/10.1016/j.femsim.2005.01.010
Immunization with glutathione S-transferase and mutant toxic shock syndrome toxin 1 fusion protein protects against Staphylococcus aureus infection

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Abstract

To investigate whether immunization with glutathione S-transferase (GST) and mutant toxic shock syndrome toxin 1 (mTSST-1) fusion protein can protect against Staphylococcus aureus infection, we purified a non-toxic mutant GST–mTSST-1 fusion protein. Mice were immunized with the GST–mTSST-1 plus alum adjuvant and then challenged with viable S. aureus. The results showed that the survival rate of GST–mTSST-1-immunized group was higher and the bacteria counts in the organs were significantly lower than those of the non-immunized mice. Immunization with GST–mTSST-1 induced strongly the production of TSST-1 specific antibodies, especially immunoglobulin G1 and immunoglobulin G2b. Furthermore, the serum samples from GST–mTSST-1-immunized mice also significantly inhibited interferon-γ and tumor necrosis factor-α production from murine spleen cells by TSST-1. These results suggest that vaccination with GST–mTSST-1 provides protection against S. aureus infection and that the protection might be mediated by TSST-1-neutralizing antibody.

Keywords: Staphylococcus aureus; Toxic shock syndrome toxin; Infection; Fusion protein; Vaccine

Journal Article.  4087 words.  Illustrated.

Subjects: Medical Microbiology and Virology ; Biotechnology ; Genetics and Genomics ; Microbiology ; Molecular and Cell Biology

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