Journal Article

A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients

Laura C. Bisio, Silvana P. Silva, Ildinete Silva Pereira, Mauro A. S. Xavier, Emerson J Venâncio, Rosana Puccia, Célia M. A. Soares and Maria Sueli S. Felipe

in Medical Mycology

Published on behalf of International Society for Human and Animal Mycology

Volume 43, issue 6, pages 495-503
Published in print September 2005 | ISSN: 1369-3786
Published online September 2005 | e-ISSN: 1460-2709 | DOI: https://dx.doi.org/10.1080/13693780400029478
A new Paracoccidioides brasiliensis 70-kDa heat shock protein reacts with sera from paracoccidioidomycosis patients

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  • Mycology and Fungi
  • Infectious Diseases
  • Medical Toxicology
  • Veterinary Medicine
  • Environmental Science

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A cDNA coding for a new member of the 70-kDa heat shock proteins (HSP70) family from the dimorphic and pathogenic fungus, Paracoccidioides brasiliensis, was cloned and characterized. The cDNA-deduced sequence coded for 655-amino acid residues and showed 95% identity to a previously described P. brasiliensishsp70 gene. Cytoplasmic and typical nuclear localization signals, which indicate induction upon stress, were identified in the deduced peptide. The complete hsp70 cDNA coding region was cloned into a pGEX 4T-3 plasmid and expressed in Escherichia coli as a glutathione-S-transferase-tagged fusion protein. The recombinant protein reacted with a rabbit polyclonal antibody against HSP70. Western immunoblot experiments demonstrated that sera from paracoccidioidomycosis patients recognized the purified recombinant protein, suggesting an immunological role for this protein in the infectious process. The antigenicity analysis of rHSP70 detected three internal peptides that could act as activators of T-cell proliferation.

Keywords: antigenic peptides; HSP70; immunoreactivity; Paracoccidioides brasiliensis; paracoccidioidomycosis; protein expression

Journal Article.  4166 words.  Illustrated.

Subjects: Mycology and Fungi ; Infectious Diseases ; Medical Toxicology ; Veterinary Medicine ; Environmental Science

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