Journal Article

Plasmid DNA Immunization against Japanese Encephalitis Virus:Immunogenicity of Membrane-Anchored and Secretory Envelope Protein

Rupinderjeet Kaur, Geetanjali Sachdeva and Sudhanshu Vrati

in The Journal of Infectious Diseases

Published on behalf of Infectious Diseases Society of America

Volume 185, issue 1, pages 1-12
Published in print January 2002 | ISSN: 0022-1899
Published online January 2002 | e-ISSN: 1537-6613 | DOI: https://dx.doi.org/10.1086/338015
Plasmid DNA Immunization against Japanese Encephalitis Virus:Immunogenicity of Membrane-Anchored and Secretory Envelope Protein

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Plasmid DNA synthesizing membrane-anchored or secretory Japanese encephalitis virus (JEV) envelope (E) protein and premembrane protein was delivered to mice by intramuscular injection or gene gun. Intramuscular plasmid immunization induced anti-E antibody responses similar to those associated with commercial JEV vaccine. The gene gun induced less antibody response. The 2 forms of the E protein induced similar antibody titers when administered by the same delivery mode. Both plasmids generated high titers of JEV-neutralizing antibodies, although the titers were lower than those induced by the vaccine. Intramuscular DNA immunization induced T helper 1 (Th1) immune responses, and the gene gun induced Th2 responses. Compared with secretory E protein, the membrane-anchored protein heavily skewed the immune response toward either Th1 or Th2, depending on the route of immunization. In an intracerebral JEV challenge model, plasmid-immunized mice had ∼60% protection; this was not affected by the form of the E protein or by immunization route

Journal Article.  9313 words.  Illustrated.

Subjects: Infectious Diseases ; Immunology ; Public Health and Epidemiology ; Microbiology

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