Chapter

Imaging molecules: radiation damage

John C. H. Spence

in High-Resolution Electron Microscopy

Published in print September 2013 | ISBN: 9780199668632
Published online January 2014 | e-ISBN: 9780191749322 | DOI: https://dx.doi.org/10.1093/acprof:oso/9780199668632.003.0006
Imaging molecules: radiation damage

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Chapter 6 is devoted to the imaging of radiation-sensitive materials, such as organic material, at high resolution using electron microscopy. The methods of single-particle imaging in cryo-electron microscopy and imaging two-dimensional crystals are described for structural biology, and additional sections review the theory of radiation damage and methods for its avoidance. The use of low voltages and tomography is also reviewed.

Keywords: radiation damage; cryo-electron microscopy; tomography; single-particle imaging; TEM

Chapter.  23251 words.  Illustrated.

Subjects: Condensed Matter Physics

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