Chapter 6 is devoted to the imaging of radiation-sensitive materials, such as organic material, at high resolution using electron microscopy. The methods of single-particle imaging in cryo-electron microscopy and imaging two-dimensional crystals are described for structural biology, and additional sections review the theory of radiation damage and methods for its avoidance. The use of low voltages and tomography is also reviewed.
Keywords: radiation damage; cryo-electron microscopy; tomography; single-particle imaging; TEM
Chapter. 23251 words. Illustrated.
Subjects: Condensed Matter Physics
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