Journal Article

Comparative analysis of the cold acclimation and freezing tolerance capacities of seven diploid Brachypodium distachyon accessions

Katia Colton-Gagnon, Mohamed Ali Ali-Benali, Boris F. Mayer, Rachel Dionne, Annick Bertrand, Sonia Do Carmo and Jean-Benoit Charron

in Annals of Botany

Published on behalf of The Annals of Botany Company

Volume 113, issue 4, pages 681-693
Published in print March 2014 | ISSN: 0305-7364
Published online December 2013 | e-ISSN: 1095-8290 | DOI: https://dx.doi.org/10.1093/aob/mct283

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  • Ecology and Conservation
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Background and Aims

Cold is a major constraint for cereal cultivation under temperate climates. Winter-hardy plants interpret seasonal changes and can acquire the ability to resist sub-zero temperatures. This cold acclimation process is associated with physiological, biochemical and molecular alterations in cereals. Brachypodium distachyon is considered a powerful model system to study the response of temperate cereals to adverse environmental conditions. To date, little is known about the cold acclimation and freezing tolerance capacities of Brachypodium. The main objective of this study was to evaluate the cold hardiness of seven diploid Brachypodium accessions.

Methods

An integrated approach, involving monitoring of phenological indicators along with expression profiling of the major vernalization regulator VRN1 orthologue, was followed. In parallel, soluble sugars and proline contents were determined along with expression profiles of two COR genes in plants exposed to low temperatures. Finally, whole-plant freezing tests were performed to evaluate the freezing tolerance capacity of Brachypodium.

Key Results

Cold treatment accelerated the transition from the vegetative to the reproductive phase in all diploid Brachypodium accessions tested. In addition, low temperature exposure triggered the gradual accumulation of BradiVRN1 transcripts in all accessions tested. These accessions exhibited a clear cold acclimation response by progressively accumulating proline, sugars and COR gene transcripts. However, whole-plant freezing tests revealed that these seven diploid accessions only have a limited capacity to develop freezing tolerance when compared with winter varieties of temperate cereals such as wheat and barley. Furthermore, little difference in terms of survival was observed among the accessions tested despite their previous classification as either spring or winter genotypes.

Conclusions

This study is the first to characterize the freezing tolerance capacities of B. distachyon and provides strong evidence that some diploid accessions such as Bd21 have a facultative growth habit.

Keywords: Brachypodium distachyon; cold acclimation; COR413; flowering; freezing tolerance; ice recrystallization inhibition; fructans; phenological development; proline; vernalization; VRN1; winter hardiness

Journal Article.  9158 words.  Illustrated.

Subjects: Ecology and Conservation ; Evolutionary Biology ; Plant Sciences and Forestry

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