Journal Article

Birth following vitrification of a small number of human oocytes: Case Report

Lilia Kuleshova, Luca Gianaroli, Cristina Magli, Anna Ferraretti and Alan Trounson

in Human Reproduction

Published on behalf of European Society of Human Reproduction and Embryology

Volume 14, issue 12, pages 3077-3079
Published in print December 1999 | ISSN: 0268-1161
Published online December 1999 | e-ISSN: 1460-2350 | DOI: https://dx.doi.org/10.1093/humrep/14.12.3077
Birth following vitrification of a small number of human oocytes: Case Report

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We report the birth of a healthy baby girl at 37 weeks gestation to a 47 year old recipient, after vitrification of mature oocytes from four in-vitro fertilization (IVF) patients. A total of 17 oocytes was vitrified in 1–2 μl of ethylene glycol (40%) and 0.6 mol/l sucrose (20.54%) in open pulled straws. Eleven oocytes survived after vitrification and five pronuclear zygotes were obtained after intracytoplasmic sperm injection (ICSI). Three embryos were transferred to three patients, two of whom were the original oocyte donors and pregnancy was not established. The third embryo was donated to a 47 year old infertile woman after preimplantation diagnosis had confirmed euploidy for chromosomes X, 13, 14, 15, 16, 18, 21 and 22. The successfully completed pregnancy is encouraging for further research to explore the potential benefits of vitrification for the cryopreservation of human oocytes, given the relatively low success of conventional freezing of human oocytes by slow cooling methods.

Keywords: birth after freezing; cryopreservation; oocyte; oocyte cryosurvival; vitrification

Journal Article.  2431 words. 

Subjects: Reproductive Medicine

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