Journal Article

The effect of gonadotrophins with differing LH/FSH ratios on the secretion of the various species of inhibin in women receiving IVF

M. Fawzy, R.F. Harrison, P.G. Knight, N. Groome, A. Anderson, W.R. Robertson and A. Lambert

in Human Reproduction

Published on behalf of European Society of Human Reproduction and Embryology

Volume 16, issue 6, pages 1092-1097
Published in print June 2001 | ISSN: 0268-1161
Published online June 2001 | e-ISSN: 1460-2350 | DOI: https://dx.doi.org/10.1093/humrep/16.6.1092
The effect of gonadotrophins with differing LH/FSH ratios on the secretion of the various species of inhibin in women receiving IVF

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We have measured secretory patterns of inhibin A, B, total α inhibin, pro-αC inhibin and oestradiol in women following pituitary suppression who were randomised into two groups to receive either urinary gonadotrophin (25:75 IU/ampoule of luteinizing hormone (LH) and follicle stimulating hormone (FSH; Normegon; n = 11) or recombinant (r)FSH (75 IU/ampoule of FSH alone, n = 16). The women were of similar age (~33 years) and length of infertility (~4 years) and had a normal endocrine evaluation. Plasma FSH, LH, oestradiol, inhibin A, B, pro-αC and total α inhibin were measured by immunoassay prior to and following gonadotrophin stimulation. Immunoactive FSH, LH and oestradiol blood concentrations following pituitary down regulation were similar in the two groups being <2.0, <3.6 IU/l and <82 pmol/l respectively. The units of FSH given (2230 versus 2764 IU; Normegon versus rFSH), duration of treatment (9.1 versus 9.4 days) and number of follicles of ≥14mm on the day of human chorionic gonadotrophin (HCG) administration (17 versus 14) were also similar. Inhibin A or B concentrations rose similarly during Normegon or rFSH administration, peaking at days 9–11. Total α and pro-αC inhibin concentrations were lower (P < 0.05) in the rFSH group during days 10 and 11 of treatment being 18.9 ± 15.9 ng/ml (Normegon) and 4.6 ± 2.8 ng/ml (rFSH) for total α inhibin and 8.5 ± 6.8 ng/ml (Normegon) and 2.8 ± 1.6 ng/ml (rFSH) for pro-αC inhibin on day 10. Overall, higher total α inhibin concentrations were associated with more mature follicles and oocytes, greater fertilization rates and better quality embryos. We conclude that inhibin A and B secretion was similar in both groups and is primarily controlled by FSH, whereas total α inhibin and pro-αC increased preferentially in the Normegon group over the rFSH group, indicating that they are, in part, stimulated by LH.

Keywords: Gonal-F; inhibin; Normegon; Puregon; recombinant FSH

Journal Article.  4896 words.  Illustrated.

Subjects: Reproductive Medicine

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