Journal Article

Aberrant expression of angiopoietins-1 and -2 and vascular endothelial growth factor-A in peri-implantation endometrium after gonadotrophin stimulation

Yin-Lau Lee, Yunao Liu, Pak-Yiu Ng, Kai-Fai Lee, Chak-Leung Au, Ernest Hung-Yu Ng, Pak-Chung Ho and William Shu-Biu Yeung

in Human Reproduction

Published on behalf of European Society of Human Reproduction and Embryology

Volume 23, issue 4, pages 894-903
Published in print April 2008 | ISSN: 0268-1161
Published online February 2008 | e-ISSN: 1460-2350 | DOI: https://dx.doi.org/10.1093/humrep/den004
Aberrant expression of angiopoietins-1 and -2 and vascular endothelial growth factor-A in peri-implantation endometrium after gonadotrophin stimulation

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BACKGROUND

Ovarian stimulation affects normal endometrial development. The expression of angiopoietin-1 (Ang-1), angiopoietin-2 (Ang-2) and vascular endothelial growth factor-A (VEGF-A) and the vascular state in the peri-implantation endometrium in women with natural and gonadotrophin-stimulated cycles were compared.

METHODS

The expression of these angiogenesis-associated molecules in endometrial biopsies, collected on Day 7 after human chorionic gonadotrophin injection or luteinizing hormone surge in stimulated or natural cycles respectively, or at mid-luteal phase of women undergoing diagnositic laparoscopy, were analysed.

RESULTS

Women with gonadotrophin-stimulation had lower Ang-1, but higher Ang-2, mRNA and protein expression (P < 0.05), and increased concentrations of von Willebrand factor (vWF) and blood vessel density than those with natural cycles (P < 0.05). Although stimulated cycles had higher VEGF-A mRNA expression (P = 0.023), VEGF-A protein expression was similar between the groups. Lower Ang-1/Ang-2 but higher Ang-2/VEGF-A mRNA ratios (P = 0.025) were found after gonadotrophin-stimulation. The ratios were negatively (P < 0.001) and positively correlated (P < 0.001) with estradiol levels, respectively. Cyclical changes in Ang-1 and Ang-2, but not in VEGF-A expression were noted.

CONCLUSIONS

The decreased Ang-1 concentration and Ang-1/Ang-2 ratio and the increased Ang-2 concentration, with the increased vWF concentration and blood vessel density, in stimulated cycles suggests advanced endometrial angiogenesis after gonadotrophin-stimulation.

Keywords: angiogenesis; angiopoietin; endometrium; ovarian stimulation; VEGF

Journal Article.  6178 words.  Illustrated.

Subjects: Reproductive Medicine

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